| Evans blue | |
|---|---|
|
tetrasodium (6E,6'E)-6,6-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(1E)hydrazin-2-yl-1-ylidene]bis(4-amino-5-oxo-5,6-dihydronaphthalene-1,3-disulfonate) |
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| Identifiers | |
| CAS number | 314-13-6 |
| PubChem | 6321418 |
| KEGG | C19422 |
| MeSH | Evans+blue |
| ChEMBL | CHEMBL1200712 |
| Jmol-3D images | Image 1 |
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| Properties | |
| Molecular formula | C34H24N6Na4O14S4 |
| Molar mass | 960.81 g mol−1 |
| (verify) (what is: /?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) |
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| Infobox references | |
T-1824 or Evans Blue, often incorrectly rendered as Evan's Blue, is an azo dye which has a very high affinity for serum albumin. Because of this, it can be useful in physiology in estimating the proportion of body water contained in blood plasma.[1]
Evans blue dye has been used as a viability assay on the basis of its penetration into non-viable cells, although the method is subject to error because damaged cells may be capable of repair[2]
Evans blue is also used to assess the permeability of the blood-brain barrier to macromolecules. Because serum albumin cannot cross the barrier, and virtually all Evans Blue is bound to albumin, normally the neural tissue remains unstained.[3] When the BBB has been compromised, albumin-bound Evans blue enters the CNS. It fluoresces with excitation peaks at 470 and 540 nm and an emission peak at 680 nm. [4]
It was named after Herbert McLean Evans, an American chemist.